Origin of Neisseria meningitidis clonal complex 4821

作者：Zhizhou Tan, Lin Xu, Jie Che, Juan Xu, Juan Xu, Li Xu, Hairui Wang, Maojun Zhang, Biao Kan, Jianguo Xu, Jianguo Xu, Zhujun Shao · 发表于：Emerging Microbes & Infections · 年份：2025 · DOI：10.1080/22221751.2025.2515461 · 被引用次数：3 · 研究领域：Bacterial Infections and Vaccines、Infective Endocarditis Diagnosis and Management、Escherichia coli research studies

Neisseria meningitidis (N. meningitidis) is the causative agent of human invasive meningococcal disease (IMD). Clonal complex (CC) 4821 is a unique genetic cluster of N. meningitidis that emerged two decades ago in Anhui Province, China and became the predominant cluster. However, the evolutionary origin of CC4821 remains unclear. Herein, a distinct CC4821 clade was identified by a comprehensive cgMLST analysis of 26,801 N. meningitidis genomes. The CC4821 clade comprised 388 N. meningitidis isolates, with 364 assigned to CC4821, 1 assigned to CC8, and 23 unassigned (UA), as they could not be assigned to any defined CC. The phylogenetic analysis of the CC4821 clade revealed that six UA isolates, including the UA isolate NmR29026 collected in 1966 from Liaoning Province, China, occupied a basal position compared to all isolates within the CC4821 clade, indicating that CC4821 originated in the 1960s. Eight subclades (clades 1 to 8) were recognized within the CC4821 clade. Clades 1 to 4 have been present since the 1970s, while clades 5 to 8 emerged after the 2000s. Clade 5 represents a hyperinvasive lineage. N. meningitidis isolate HEB85-3, collected in 1985 in Hebei Province, China, exhibited the closest evolutionary relationship to clade 5, suggesting it is related to the origin of this hyperinvasive lineage. Our study reveals that CC4821 has emerged as the predominant cluster of N. meningitidis in China, representing the culmination of at least 60 years of continuous evolutio...