Photothermal-Responsive Conjugated Polymer Nanoparticles Triggered Precise Augmentation of Microalgal Photosynthesis

作者：Miaomiao Zhang, Chenying Gao, Pengcheng Wang, Rui Li, Yiming Huang, Fengting Lv, Cheng Li, Haotian Bai, Deqing Zhang, Shu Wang · 发表于：ACS Nano · 年份：2025 · DOI：10.1021/acsnano.5c06705 · 被引用次数：8 · 研究领域：Algal biology and biofuel production、Advanced Nanomaterials in Catalysis、TiO2 Photocatalysis and Solar Cells

Exploring precise temperature-based photosynthesis regulation strategies will afford promising opportunities for controllable and efficient enhancement of photosynthesis. In this work, we developed a type of photothermal conjugated polymer nanoparticles (T-NPs) based on TZQ-TDPP (poly[2,5-bis(2-ethylhexyl)-3,6-di(thiophen-2-yl)-2,5-dihydropyrrolo[3,4- c ]pyrrole-1,4-dione- co -(6,7-bis(4-((2-decyltetradecyl)oxy)phenyl) -[1,2,5] thiadiazolo[3,4-g]quinoxaline)]). T-NPs possess the desired photothermal conversion performance, with their photothermal conversion efficiency reaching up to 80.52%. The introduction of T-NPs enables the controllable regulation of the microenvironment temperature of Chlorella pyrenoidosa ( C. pyre ), thereby achieving accurate regulation on its photosynthetic activity. Under optimal conditions, T-NPs accelerate light-dependent reactions, leading to increased oxygen evolution and NADP +, NADPH, and ATP contents up to 46%, 20%, 23%, and 103%, respectively. Moreover, the Rubisco activity of C. pyre under light-independent reactions increased by 258%. The chlorophyll fluorimeter parameters and chlorophyll fluorescence kinetics curves also exhibited varying degrees of increase, demonstrating that the introduction of T-NPs improves the photosystem II (PSII)-related process and enhances the photosynthesis process of C. pyre . Integrated proteomic profiling revealed the regulatory mechanism of T-NPs on the photosynthesis of C. pyre and confirmed the upregulati...