PGE2 Ameliorates Aging-Aggravated Rotator Cuff Muscle Atrophy

作者：Longqiang Shu, Xin Wang, Haoyuan Wang, Yang Tang, Chongyang Wang, Yao Zhang · 发表于：Journal of Bone and Joint Surgery · 年份：2025 · DOI：10.2106/jbjs.24.00866 · 被引用次数：2 · 研究领域：Shoulder Injury and Treatment、Chronic Obstructive Pulmonary Disease (COPD) Research、Muscle Physiology and Disorders

BACKGROUND: The aging-related escalation of muscle degeneration impacts the structure and function of rotator cuff muscles, contributing to spontaneous and tear-induced muscle atrophy. This study investigated how prostaglandin E2 (PGE2), a regulator of muscle regeneration, influences muscular structure and mitochondrial function in aged mice by using SW033291 to inhibit PGE2 degradation, revealing potential therapeutic pathways for mitigating rotator cuff muscle deterioration. METHODS: A total of 20 young (5 to 6-month-old) and 100 aged (18 to 20-month-old) female C57BL/6J mice were divided into 2 groups: the first group included young, aged, and aged+SW033291 subgroups and was used to study sarcopenia, and the second group consisted of tear, tear+repair, and tear+repair+SW033291 subgroups and was used to examine the outcomes following a rotator cuff tear (RCT). Tissue staining, muscle mass assessments, functional assays, and mitochondrial function tests were performed. RESULTS: Rotator cuff muscle degeneration was observed in the setting of natural aging and in the setting of an RCT. These conditions together worsened muscle atrophy and fatty infiltration into the muscle, with the aged tear group demonstrating a decrease in muscle mass from a mean and standard deviation of 45.45 ± 4.04 to 25.18 ± 1.82 mg (p < 0.001) and a reduction in fiber cross-sectional area (CSA) from 1,697.3 ± 108.4 to 1,263.0 ± 56.8 μm 2 (p < 0.001). This was linked to increased 15-prostaglandin dehydr...