Hyaluronic acid‐functionalized nanoarmor enhances the stable colonization ability of Paenibacillus polymyxa JF_P68 and boosts its biological control efficacy against pear anthracnose

作者：Jianfeng Du, Heng Li, Shenjie Liu, Limin Wu, Yiming Liu, Yue Li, Xin Zhao, Qixiong Gao, Jing Dong, Lei Chen, Fuxin Sun, Xueliang Tian, Chuanjun Wang, Ninghai Lu, Yang Jiao · 发表于：Pest Management Science · 年份：2025 · DOI：10.1002/ps.8868 · 被引用次数：4 · 研究领域：Plant Surface Properties and Treatments、Plant-Microbe Interactions and Immunity、Postharvest Quality and Shelf Life Management

BACKGROUND: The application of biocontrol agents offers a promising strategy for managing pear anthracnose, yet their efficacy is often limited by unstable colonization on fruit surfaces. RESULTS: Here, we isolated Paenibacillus polymyxa JF_P68, which exhibits strong inhibitory activity against Colletotrichum fructicola. To enhance its environmental tolerance, we developed a hyaluronic acid (HA)-functionalized nanoarmor using a layer-by-layer coating technique. This nanoarmor significantly improved the JF_P68's resistance to ultraviolet radiation and oxidative stress. In vivo trials demonstrated that nanoarmor-equipped JF_P68 reduced pear anthracnose lesion diameters by 43.5% and maintained higher colonization levels on fruit surfaces. Genome analysis revealed 20 biosynthetic gene clusters, including novel antimicrobial candidates, underpinning its broad-spectrum antifungal activity. CONCLUSION: The single-cell coating method, characterized by simplicity, scalability, and biocompatibility, significantly enhances the persistence and efficacy of biocontrol agents. This study provides a novel approach to improving the biological control of pear anthracnose. © 2025 Society of Chemical Industry.