Correlations among Antibacterial Efficacy in Dentinal Tubules, pH, and Calcium Ion Release of 5 Premixed Calcium Silicate-based Sealers in a Novel ex vivo Model

作者：He Liu, Chanchan Chen, Zhejun Wang, Ya Shen · 发表于：Journal of Endodontics · 年份：2025 · DOI：10.1016/j.joen.2025.05.026 · 被引用次数：11 · 研究领域：Endodontics and Root Canal Treatments、Dental materials and restorations、Dental Erosion and Treatment

Introduction To evaluate the antibacterial efficacy of five premixed calcium silicate-based sealers in infected dentinal tubules using a novel ex vivo model, and to investigate correlations among antibacterial efficacy, pH, and calcium ion release. Methods Forty human premolars with a single canal were selected and stored in 0.01% NaOCl solution at 4 °C. The teeth were sectioned to obtain 64 dentin blocks, which were sterilized and inoculated with Enterococcus faecalis for 3 weeks. Four infected blocks were analyzed using scanning electron microscopy. Sixty infected blocks were fitted into 3D-printed teeth and assigned to six groups based on the sealer used: Control, BIO-C SEALER ION + (BIOC + ), EndoSequence BC Sealer HiFlow (HF), EndoSequence BC Sealer (BC), NeoSEALER Flo (NEO), and BioRoot Flow (BRF). Specimens were subsequently obturated using the single-cone technique. Antibacterial efficacy was evaluated at 1 and 4 weeks using viability staining and confocal laser scanning microscopy. Leachates from sealer discs were collected to analyze pH and calcium ion at day 1, 1 week, and 4 weeks. Statistical analysis was performed using one-way ANOVA, unpaired t-test, and Spearman's correlation (α = 0.05). Results At 1 and 4 weeks after obturation, no significant differences in antibacterial efficacy were observed among the sealer groups; all groups exhibited significantly higher antibacterial efficacy at 4 weeks compared to 1 week ( P < .05). At day 1, 1 week, and 4 weeks, all s...