Fast-Response Mitochondria-Targeted Fluorescent Probe for H 2 S Imaging in Tumors and Inflammation

作者：Huixiang Wang, Erting Feng, Huizhen Ma, Fangfang Du, Muyang Chen, Zongjin Qu, Fabiao Yu · 发表于：Chemical & Biomedical Imaging · 年份：2025 · DOI：10.1021/cbmi.5c00046 · 被引用次数：11 · 研究领域：Sulfur Compounds in Biology、Nanoplatforms for cancer theranostics、Eicosanoids and Hypertension Pharmacology

High Resolution Image Download MS PowerPoint Slide In order to explore the complex interaction between H 2 S and the development of related diseases, it is urgently necessary to develop effective visualization methods to monitor the dynamic changes of H 2 S in real time. Herein, we constructed the NIR fluorescent probe HCy-SSPy based on disulfide cleavage for the rapid imaging of H 2 S. The hemicyanine (HCy-NH 2 ) unit was used as a NIR fluorophore, and asymmetric pyridyl disulfides (SSPy) acted as the specific recognition receptor for H 2 S. The synthesized HCy-SSPy showed a remarkable NIR turn-on signal at 765 nm activated by H 2 S. This probe also possessed excellent selectivity and high sensitivity, as well as rapid detection ability for H 2 S (∼5 s). Moreover, the low cytotoxicity, mitochondrial localization, and excellent cell imaging performance of HCy-SSPy were discussed. Further biological experiments revealed that the probe not only imaged H 2 S in tumor-bearing mice but also showed great potential for H 2 S detection in inflammatory processes.