How do antibiotic residues in manure affect grassland plants and soil nitrogen cycling?

作者：Zhongchen Yang, Youssef C. Wang-Touri, Jan Willem van Groenigen, B.J.A. Berendsen, Milou G.M. van de Schans, Gerlinde B. De Deyn · 发表于：Plant and Soil · 年份：2025 · DOI：10.1007/s11104-025-07589-y · 被引用次数：4 · 研究领域：Pharmaceutical and Antibiotic Environmental Impacts、Pesticide and Herbicide Environmental Studies

Abstract Background and aims Animal manure is a valuable fertilizer, and its proper use is essential in circular agriculture. However, antibiotics are commonly administered to livestock and excreted in manure, thereby entering soil ecosystems. The effects of antibiotic-containing manure on soil nitrogen (N) cycling microbial guilds, plant productivity, and N turnover in grassland ecosystems remain unclear. Methods In a two-factorial greenhouse pot experiment, we evaluated the impact of manure with different antibiotic residues in four plant communities: grass monoculture, clover monoculture, grass-clover culture, and a no-plant control. The fertilization treatments included antibiotic-free manure, manure containing oxytetracycline, and manure containing sulfadiazine. We measured soil N-cycling functional genes, aboveground and belowground plant biomass, clover symbiotic N fixation, soil mineral N pools, N 2 O emissions, and antibiotic residues in plants and soil. Results Oxytetracycline, but not sulfadiazine, significantly increased the relative abundance of ammonia-oxidizing archaea (AOA) and marginally increased the abundance of N-fixing microbes across all plant communities. In clover monoculture, both antibiotics reduced root biomass and root total N content. However, antibiotic residues in soil did not have significant impacts on N fixation of clover, soil mineral N pools, and soil N 2 O emissions. Conclusion At environmentally relevant concentrations, oxytetracycline re...