Enzymatic Modulating Zinc-Air Battery-Based Self-Powered Sensor Coupling Photoactive Covalent Organic Framework for Organophosphorus Pesticide Detection

作者：Xianzhou Cai, Qian Cui, Ting Bao, Wei Wen, Xun Zhang, Shengfu Wang · 发表于：Analytical Chemistry · 年份：2025 · DOI：10.1021/acs.analchem.5c02248 · 被引用次数：8 · 研究领域：Electrochemical sensors and biosensors、Advanced Nanomaterials in Catalysis、Conducting polymers and applications

Self-powered electrochemical sensors (SPES) have gained significant attention with the characteristics of portability, high sensitivity, and no requirement of an external power supply. Herein, a novel photoassisted zinc-air battery-based SPES (PZAB-SPES) was developed for organophosphorus pesticide detection based on photoactive covalent organic framework (COF) and enzymatic modulation strategy. The in situ grown COF nanosheets not only acted as the photocathode but also served as nanocarriers of silver nanoparticles (AgNPs) to improve the photoelectric conversion efficiency with the surface plasmon resonance effect, which markedly increased the open-circuit voltage up to 1.469 V. Based on the aptamer-target recognition and efficient separation of magnetic beads, the alkaline phosphatase (ALP)-catalyzed reaction was triggered, resulting in the hydrolysis of sodium thiophosphate (TP) and the generation of hydrogen sulfide (H 2 S). The generated H 2 S caused the oxidation of AgNPs to form silver sulfide (Ag 2 S), which decreased the photoelectric conversion efficiency and inhibited the oxygen reduction reaction, leading to a decrease of the open-circuit voltage. As a proof of concept, the proposed PZAB-SPES exhibited good analytical performance for acetamiprid detection ranging from 0.01 to 1000 ng/mL, with a low detection limit of 1.1 pg/mL. This fascinating sensing platform provided an efficient method for organophosphorus pesticide detection, holding extensive application pr...