RNAi-Mediated Silencing of Chitin Synthase 1 (CHS1) Disrupts Molting and Growth in Monochamus alternatus

作者：Wanlin Ye, Tong Li, Mingqing Weng, Wenchi Guo, Fei-Yi Xin, Wei Yu, Songqing Wu, Yajie Guo · 发表于：Forests · 年份：2025 · DOI：10.3390/f16060922 · 被引用次数：5 · 研究领域：Studies on Chitinases and Chitosanases、Aquaculture Nutrition and Growth、Invertebrate Immune Response Mechanisms

Chitin synthase (CHS) plays a key role in chitin synthesis. CHS1 is ubiquitous in insects, and some studies have found that the RNA interference with CHS1 can hinder three types of molting processes (larva–larva, larva–pupa and pupa–adult). In the present study, the CHS1 of Monochamus alternatus was identified and characterized by a bioinformatics analysis. The developmental stage-specific expression of the MalCHS1 (Monochamus alternatus CHS1) gene was obtained by a RT-qPCR, and the corresponding dsRNA was designed for functional verification. The RNA interference experiment was conducted using the microinjection method, and the injection site was selected from the abdominal segments of fifth-instar larvae. The results showed that after silencing the CHS1 gene, the larvae of M. alternatus showed morphological abnormalities, such as the softening of the body wall, a transparent abdomen and the swelling of somites, indicating that MalCHS1 mediates the molting, growth and development of M. alternatus. RNAi-mediated MalCHS1 gene silencing may become a promising new biological pesticide that can provide a new target gene for pest control.