Pharmacological effects and mechanisms of Xuanfei Baidu Decoction in the treatment of viral pneumonia

作者：Jingsheng Zhang, Bo Pang, Qiyue Sun, Jing Sun, Shan Cao, Yingli Xu, Yu Zhang, Xinqi Deng, Shanshan Guo, Lei Bao, Zihan Geng, Shuran Li, Ronghua Zhao, Daohan Wang, Xiao-Lan Cui, Bin Qu, Yu Tian Wang · 发表于：Science of Traditional Chinese Medicine · 年份：2025 · DOI：10.1097/st9.0000000000000072 · 被引用次数：2 · 研究领域：Calcium signaling and nucleotide metabolism、Andrographolide Research and Applications、Chronic Obstructive Pulmonary Disease (COPD) Research

Abstract Objective This study aims to investigate the therapeutic effects and underlying mechanisms of Xuanfei Baidu Decoction (XFBD) in a mouse model of dampness-heat toxin pneumonia. By exploring how XFBD exerts its effects, we seek to deepen our understanding of its role in treating pulmonary diseases and to address the current knowledge gap regarding its mechanisms of action, thereby supporting its clinical application. Methods Ultra-high-performance liquid chromatography and high-resolution mass spectrometry (HRMS) were employed to analyze the chemical constituents of XFBD. The protective effects of XFBD were evaluated using a dampness-heat toxin-induced mouse model, established through dampness-heat exposure and HCoV-229E infection. XFBD was administered orally, followed by assessments including lung index measurement, micro-CT imaging, viral load quantification, cytokine analysis, and histological evaluation via hematoxylin-eosin staining. Proteomics and single-cell transcriptomic analyses were conducted to explore the potential mechanisms underlying XFBD’s pharmacological effects. A cellular model of HCoV-229E infection was developed to investigate changes in the cAMP/PKA signaling pathway. Molecular docking and surface plasmon resonance (SPR) experiments confirmed the strong binding affinity between key XFBD components and PKA. Finally, PKA activators and inhibitors were applied in vitro to validate these mechanistic findings. Results In vivo studies demonstrated tha...