Best-practice guidance for Earth BioGenome Project sample collection and processing: progress and challenges in biodiverse reference genome creation

作者：Mara Lawniczak, Kevin M. Kocot, Jonas J. Astrin, Mark Blaxter, Cibele G. Sotero-Caio, Katharine Barker, Anna K. Childers, Jonathan A. Coddington, Paul A. Davis, Kerstin Howe, Warren E. Johnson, Duane D. McKenna, Jeremy G. Wideman, Olga Vinnere Pettersson, Verena Ras, Bernardo F. Santos, Mara Lawniczak, Jonas J. Astrin, Anna K. Childers, Kevin M. Kocot, Duane D. McKenna, Olga Vinnere Pettersson, Verena Ras, Bernardo F. Santos, Jeremy G. Wideman · 发表于：GigaScience · 年份：2025 · DOI：10.1093/gigascience/giaf041 · 被引用次数：10 · 研究领域：Genomics and Phylogenetic Studies、Genetics, Bioinformatics, and Biomedical Research、Molecular Biology Techniques and Applications

The Earth BioGenome Project has the extremely ambitious goal of generating, at scale, high-quality reference genomes across the entire Tree of Life. Currently in its first phase, the project is targeting family-level representatives and is progressing rapidly. Here we outline recommended standards and considerations in sample acquisition and processing for those involved in biodiverse reference genome creation. These standards and recommendations will evolve with advances in related processes. Additionally, we discuss the challenges raised by the ambitions for later phases of the project, highlighting topics related to sample collection and processing that require further development.