Capillary Force-Driven Capture of Magnetic Nanoparticles in Calcium Phosphate Hollow-Tube Whisker Scaffolds for Osteonecrosis of the Femoral Head

作者：Yi Zhou, Cong Feng, Xiaolong Yang, Yu Jiang, Xiangfeng Li, Weili Fu, Xiangdong Zhu, Jian Li, Xingdong Zhang · 发表于：ACS Nano · 年份：2025 · DOI：10.1021/acsnano.5c02874 · 被引用次数：13 · 研究领域：Bone and Joint Diseases、Erythrocyte Function and Pathophysiology、Extracellular vesicles in disease

Excessive glucocorticoid use disrupts osteogenesis and angiogenesis in the femoral head, leading to steroid-induced osteonecrosis of the femoral head (SONFH), which is a significant clinical challenge. This study introduces a magnetically responsive biphasic calcium phosphate (HBCP/Fe 3 O 4 ) scaffold featuring a nanoparticle-embedded hollow-tube whisker structure. The scaffold was fabricated through an in situ growth process to generate hollow-tube whiskers, followed by a capillary trapping technique that allowed the hollow-tube whiskers to capture Fe 3 O 4 nanoparticles (NPs), achieving uniform and efficient encapsulation. HBCP/Fe 3 O 4 exhibited excellent magnetic responsiveness and significant biological effects under static magnetic field (SMF) stimulation. In vitro, HBCP/Fe 3 O 4 under SMF promoted osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in a glucocorticoid microenvironment, enhanced angiogenesis in human umbilical vein endothelial cells (HUVECs), and induced M2 polarization of RAW 264.7 murine macrophage cells (RAW 264.7). Furthermore, HBCP/Fe 3 O 4 under SMF stimulation orchestrated paracrine signaling from endothelial and immune cells, thereby enhancing the osteogenic differentiation of BMSCs. Mechanistically, the osteogenic differentiation of BMSCs was driven by magnetic stimulation-induced Piezo1-mediated Ca 2+ influx, which activated BMP-2/Smad signaling and upregulated key osteogenic markers. In vivo, the implantation of HBCP/Fe 3...