Lian Qiao Wen Dan Decoction attenuates gastric carcinogenesis by alleviating oxidative phosphorylation dysfunction

作者：Xiaochun Chi, Yanru Song, Wei Sisi, Guangjun Wang, Wen Yang, Zhe Zhang, Cong Zhang, Hongtao Zhang, Zhongdong Hu, Haiyang Yu, Bingjie Huo, Baoen Shan, Lianmei Zhao · 发表于：Phytomedicine · 年份：2025 · DOI：10.1016/j.phymed.2025.156924 · 被引用次数：4 · 研究领域：Metabolism, Diabetes, and Cancer、Cancer, Hypoxia, and Metabolism、Metabolomics and Mass Spectrometry Studies

Background Wen Dan Decoction (WDD) is primarily utilized as an “expectorant” in clinical practice. The combination of WDD and Lian Qiao (LQWDD) exhibits synergistic effects on phlegm resolution, qi regulation, and detoxification. In preliminary clinical practice, we observed that LQWDD effectively alleviates the severity of gastric precancerous lesions. Nevertheless, the underlying mechanism remains elusive. Purpose This study was designed to explore the efficacy and potential mechanisms of LQWDD against gastric carcinogenesis. Methods Retrospective analyses of patients with gastric precancerous lesions and an N-methyl-N9-nitro-N-nitrosoguanidine (MNNG)-induced rat gastric cancer model were conducted to evaluate the efficacy of LQWDD against gastric carcinogenesis. Proteomic analyses were employed to investigate the underlying mechanisms of LQWDD. Moreover, energy metabolites were quantified using metabolomics. Network pharmacology and high-performance liquid chromatography-mass spectrometry were integrated to elucidate the potential active components of LQWDD. Additionally, seahorse analysis and RNA sequencing were used to explore the effects and underlying mechanisms of quercetin on oxidative phosphorylation. Results LQWDD effectively impeded gastric carcinogenesis in an MNNG-induced rat gastric cancer model. Proteomic analyses revealed that LQWDD protected the integrity of the oxidative phosphorylation pathway during gastric carcinogenesis, which was significantly suppress...