Genomic characteristics and epidemic trends of NADC30-like PRRSV in China

作者：Siyu Zhang, Xu Hu, Zhenyang Guo, Lirun Xiang, Chao Li, Bangjun Gong, Jinhao Li, Zixuan Feng, Haonan Kang, Qian Wang, Guohui Zhou, Chaoliang Leng, Kuan Zhao, Yan‐Dong Tang, Huairan Liu, Tongqing An, Xuehui Cai, Jinmei Peng, Zhijun Tian, Hongliang Zhang · 发表于：Porcine Health Management · 年份：2025 · DOI：10.1186/s40813-025-00444-7 · 被引用次数：14 · 研究领域：Animal Virus Infections Studies、SARS-CoV-2 and COVID-19 Research、Polyomavirus and related diseases

BACKGROUND: NADC30-like PRRSV was first identified in China in 2012 and had become the predominant circulating strain since 2016. Currently, the recombination patterns of NADC30-like PRRSV in China exhibit a high degree of complexity, characterized by low whole-genome sequence homology. The genomic features and epidemiological trends of these strains remain to be elucidated. RESULTS: To evaluate the prevalence of NADC30-like PRRSV in China, this study acquired 30 whole-genome sequences of NADC30-like strains via Next-Generation Sequencing (NGS). These sequences were subsequently integrated with 224 whole-genome sequences from China available in the GenBank database. A comprehensive analysis of the genomic characteristics of contemporary NADC30-like PRRSV strains in China was conducted. Recombinant analysis indicated a yearly increase in the number of NADC30-like strains exhibiting recombination signals, whereas nonrecombinant NADC30-like strains have become nearly extinct. Among the recombination events, those involving L1C and L8E as parental strains are most prevalent. Based on the results of recombination and phylogenetic analyses, this study classified 120 Chinese NADC30-like strains with similar recombination characteristics into groups NADC30-R1 to R12. The intra-group genetic distances of the NADC30-R1 to R12 groups approximately 5.73% (SD ± 1.68), while the inter-group genetic distances between different groups are usually stably greater than 10%. The amino acid align...