Long-term in vivo monitoring of transplanted mesenchymal stromal cells in colitis mice with magnetic particle imaging

作者：Tuo Shao, Zelin Gu, Yuzhu Liu, Xiaoyi Wang, Chao Tang, Nannan Chen, Mengqi Wang, Xueqin Liu, Huanhuan Song, Siye Chen, Lin Li, Hui Hui, Xiaohua Jia, Hui Mao, Raymond T. Chung, Steven H. Liang, Sidong Xiong, Jie Tian · 发表于：EBioMedicine · 年份：2025 · DOI：10.1016/j.ebiom.2025.105775 · 被引用次数：8 · 研究领域：Characterization and Applications of Magnetic Nanoparticles、Nanoparticle-Based Drug Delivery、Inflammatory Bowel Disease

BACKGROUND: The successful development of mesenchymal stromal cells (MSCs)-based therapeutic strategies in inflammatory bowel disease (IBD) hinges on the ability to assess the influence of the administration route, while also tracking the cells' in vivo location, distribution, and long-term viability. Moreover, understanding the biological fate of these cells-particularly their activation, differentiation, and interaction within the host environment-is crucial to optimising their therapeutic potential. METHODS: This study explores the use of magnetic particle imaging (MPI) with superparamagnetic iron oxide nanoparticles (SPIONs) to track MSCs in colitis mice. SPION-labelled MSCs were administered through intravenous (IV), intraperitoneal (IP), and intrarectal (IR) routes. MPI was employed to track MSCs. FINDINGS: MPI provided dynamic tracking of MSC distribution, viability, and homing, revealing that IP injections led to more targeted and sustained delivery to inflamed colon tissues, with higher cell survival and efficacy in modulating inflammation. Other administration routes also demonstrated specific distribution patterns, but with shorter retention times. The prolonged survival of MSCs following IP injection may be attributed to the immune environment in the peritoneal cavity, which significantly impacts MSCs, influencing their survival and migration. Further analyses showed that MSCs exposed to inflammatory peritoneal fluid exhibited enhanced homing capabilities, and IFN...