H2O2‐Dependent Methyl Jasmonate Regulates H2S‐Induced Resistance to Fusarium oxysporum f. sp. niveum Race 2 in Citrullus lanatus

作者：Shiyu Li, Tongshu Zhao, Ning Chang, Yi Chen, Qi Wang, Zhongyuan Wang, Chunhua Wei, Jianxiang Ma, Yong Zhang, Xian Zhang, Hao Li · 发表于：Plant Cell & Environment · 年份：2025 · DOI：10.1111/pce.15654 · 被引用次数：6 · 研究领域：Nematode management and characterization studies、Plant Disease Management Techniques、Potato Plant Research

ABSTRACT Fusarium wilt, caused by Fusarium oxysporum (Fo), is a destructive fungal disease that reduces crop yield and quality. Hydrogen sulphide (H 2 S), a critical signalling molecule, modulates plant defence responses; however, its role and mechanism in combating Fo remain elusive. This study reveals that exogenous NaHS (an H 2 S donor) enhances watermelon resistance to Fusarium oxysporum f. sp. niveum race 2 (FON2), accompanied by elevated hydrogen peroxide (H 2 O 2 ) and methyl jasmonate (MeJA) levels. Exogenous H 2 O 2 and MeJA also enhance FON2 resistance. Conversely, silencing respiratory burst oxidase homologue F ( ClRBOHF ) and jasmonic acid carboxyl methyltransferase ( ClJMT ), key genes for H 2 O 2 and MeJA biosynthesis, respectively, inhibits NaHS‐induced resistance to FON2. Deletion of l ‐cysteine desulfhydrase ( ClLCD ), a pivotal gene for H 2 S generation, reduces FON2 resistance, but this reduction is restored by H 2 O 2 or MeJA supplementation. Upon FON2 infection, exogenous H 2 O 2 elevates MeJA levels; however, silencing ClRBOHF suppresses NaHS‐induced MeJA accumulation. Furthermore, silencing ClClJMT inhibits H 2 O 2 ‐induced FON2 resistance, while MeJA supplementation rescues the reduced resistance caused by ClRBOHF silencing. Collectively, these findings demonstrate that H 2 O 2 ‐dependent MeJA plays a crucial role in regulating H 2 S‐induced watermelon resistance to FON2. The growing focus on reducing pesticide use highlights the potential of this mech...