Comparative efficacy of single vs. double autologous platelet-rich plasma ovarian injections for improving ovarian response in poor ovarian responders

作者：Qian Li, Jiayi Guo, Jiawen Liu, Jing Li, Lina Zou, Cong Fang, Xiaoyan Liang · 发表于：Scientific Reports · 年份：2025 · DOI：10.1038/s41598-025-02689-2 · 被引用次数：6 · 研究领域：Ovarian function and disorders、Sperm and Testicular Function、Reproductive Biology and Fertility

Poor ovarian response (POR) is a challenge in reproductive medicine, often leading to suboptimal outcomes in IVF/ICSI cycles. Autologous platelet-rich plasma (PRP) injections have emerged as a potential therapy to enhance ovarian function. This study aims to compare the efficacy of single versus double PRP ovarian injections in improving ovarian reserve, response to stimulation, and IVF/ICSI outcomes in women with POR. This before-and-after study was conducted at the Reproductive Center of the Sixth Affiliated Hospital of Sun Yat-sen University. Seventy-one women diagnosed with POR (POSEIDON group 3 or 4), characterized by anti-Müllerian hormone (AMH) levels below 1.2 ng/mL and fewer than five antral follicles, who had completed at least one IVF/ICSI cycle before and after PRP treatment were included. Participants received intraovarian injections of autologous PRP (2-2.5 mL per ovary) via transvaginal ultrasound guidance, either once or twice as determined clinically. We evaluated changes in ovarian reserve markers (AMH and antral follicle count [AFC]) and IVF/ICSI outcomes, such as the number of retrieved oocytes and quality embryos. Comparative analysis between single and double injections utilized the difference (Δ) between post- and pre-treatment values. PRP treatment resulted in significant improvements in AMH levels (from 0.33 ± 0.24 ng/mL to 0.43 ± 0.29 ng/mL, p = 0.005) and AFC (from 2.62 ± 1.09 to 3.80 ± 1.95, p < 0.001). Both single and double PRP injections signifi...