Poliumoside alleviates microglia-mediated inflammation and blood-brain barrier disruption via modulating the polarization of microglia after ischemic stroke in mice

作者：Yuxiao Gao, Jingjing Wang, Cong Zhang, Huan Wang, Bin Wang, Xiangjian Zhang · 发表于：Phytomedicine · 年份：2025 · DOI：10.1016/j.phymed.2025.156881 · 被引用次数：19 · 研究领域：Neuroinflammation and Neurodegeneration Mechanisms、Neurological Disease Mechanisms and Treatments、Barrier Structure and Function Studies

BACKGROUND: Ischemic stroke remains a life-threatening condition with limited therapeutic options. Microglia-mediated neuroinflammation critically exacerbates acute ischemic injury. The active compound poliumoside (Pol) in Callicarpa kwangtungensis Chun exhibits significant anti-inflammatory effects. The therapeutic potential of Pol for ischemic stroke remains unknown and promising. PURPOSE: The current study aimed to investigate the effect of Pol on acute ischemic stroke in mice, and to elucidate the underlying mechanisms. STUDY DESIGN AND METHODS: Ischemic stroke mice model was induced by transient middle cerebral artery occlusion model (tMCAO). Pol was administered by intraperitoneal injection after stroke. Neurological deficits were monitored up to 3 days after stroke. Microglial polarization, and microglia-associated inflammatory cytokines and blood-brain barrier (BBB) integrity were detected in the peri‑infarct cortex at day 1 after stroke. RNA-seq analysis was performed to identify potential pathways recruited by Pol. Primary cortical neuron, BV2 microglia cell lines and mouse brain microvascular endothelial cell lines(bEnd.3) were employed to explore the underlying mechanism in vitro. RESULTS: Compared with the tMCAO group, Pol significantly alleviated neurological deficits and reduced infarct size in mice. In vitro and in vivo experiments demonstrated that Pol regulates microglial polarization, down-regulates inflammatory factor levels (IL-6 and TNF-α), and attenuate...