NMB promotes the progression of colorectal cancer by regulating the NF-κB/P65 signaling pathway

作者：Jiaxin Fan, Yuming Liu, Jiajia Wang, Yao Tian, Shaoxiong Bai, Xiaole Ma, Hao Chen, Yuhao Chen, Huiyang Gao, Yuntong Guo, He Huang · 发表于：Frontiers in Immunology · 年份：2025 · DOI：10.3389/fimmu.2025.1596451 · 被引用次数：5 · 研究领域：NF-κB Signaling Pathways、Neuropeptides and Animal Physiology、Cancer, Stress, Anesthesia, and Immune Response

Background Colorectal carcinoma (CRC) ranks as the fourth most prevalent malignancy globally. Neuromedin B (NMB) and its corresponding receptor have been implicated in the pathogenesis of multiple neoplastic conditions. Nevertheless, the specific involvement of NMB in CRC progression remains poorly characterized. Methods To investigate the differential expression of NMB, data were extracted from the TCGA and GEO databases, supplemented by tissue microarrays derived from clinical specimens. Prognostic significance was assessed through Kaplan-Meier survival analysis, ROC curve evaluation, Cox proportional hazards regression, clinical correlation studies, and nomogram construction. Experimental validation of NMB expression in CRC was performed using qRT-PCR, western blotting, immunohistochemistry, and ELISA techniques. Functional characterization of NMB's tumor-regulatory capacity was conducted through colony formation assays, MTT proliferation tests, wound healing experiments, and transwell migration/invasion assays. Mechanistic insights were obtained by identifying upstream regulatory proteins via co-immunoprecipitation and exploring potential signaling pathways through gene set enrichment analysis (GSEA) combined with western blot validation. Results Analysis of clinical data revealed that NMB exhibits markedly elevated expression levels in CRC tissues, with higher expression correlating significantly with poor prognosis. Receiver operating characteristic curve analysis valid...