Enhanced Antibacterial and Anti‐Inflammatory Efficiency of Serratiopeptidase Immobilized on CMC‐Silver Nanoparticles

作者：Taleeha Roheen, Mehvish Bibi, Muhammad Fayyaz ur Rehman, Nasir Assad, Humaira Yasmeen Gondal, Muhammad Nadeem, Farhan Ahmad Atif, Fozia Batool, Nazia Perveen, Rahman Qadir, Saif Ur Rehman, Misbah Maqbool, Shahzad · 发表于：Journal of Basic Microbiology · 年份：2025 · DOI：10.1002/jobm.70060 · 被引用次数：11 · 研究领域：Pineapple and bromelain studies、Enzyme Production and Characterization、Peptidase Inhibition and Analysis

ABSTRACT In the present study, sunlight‐mediated Carboxymethyl cellulose silver nanoparticles (CMC‐AgNPs) have been synthesized as a carrier for serratiopeptidase immobilization. Morphological behavior of CMC‐AgNPs, crystallinity and functional group identification were evaluated using scanning electron microscopy, X‐ray diffraction and Fourier transform infrared spectroscopy, respectively. The prepared nanoparticles (NPs) were also subjected to zeta potential, which disclosed the zeta potential value of about—36.06 mV, suggesting their negative charge surface, good stability and polydispersity. SRP was immobilized on synthesized NPs through covalent adsorption using glutaraldehyde as a crosslinker. Immobilized (CMC/Ag‐SRP) exhibited 80.95% immobilization efficiency and 79.73% immobilization yield, respectively. Remarkably greater relative activities at broader temperature and pH ranges were attained by SRP after immobilization in comparison to its free counterpart. The K m value was significantly higher for immobilized enzyme, whereas V max value was conspicuously lower, indicating that less enzyme was sufficient to achieve maximum velocity. The greater zone of inhibition was displayed by immobilized CMC‐AgNPs than that of native NPs against both gram‐positive Listeria monocytogenes (12 ± 0.05 mm) and gram‐negative Escherichia coli (22 ± 0.12 mm). The bigger zone on casein agar plates for immobilized NPs confirms enhanced caseinolytic activity in comparison to starting mater...