Correlation of the intestinal flora and its metabolites with the colonic transport function in functional constipation

作者：Lunan Hu, Qihong Liu, Xiao Ke, Peilin Zhao, Wenyi Fang, Yan Ren · 发表于：Frontiers in Microbiology · 年份：2025 · DOI：10.3389/fmicb.2025.1591697 · 被引用次数：9 · 研究领域：Gastrointestinal motility and disorders、Gut microbiota and health、Probiotics and Fermented Foods

Background Functional constipation (FC) is a clinically frequent intestinal disorder. A growing body of evidence emphasizes the link between intestinal microecological imbalance and constipation. However, the microbiota composition associated with FC and the mechanisms by which metabolites influence gut motility remain poorly understood. Methods Stool samples were collected from 60 participants (20 FC patients with delayed colonic transit time, 20 FC patients with normal colonic transit time, and 20 healthy controls), and macrogenomics and metabolomics were used to assess the differences in the microbiota and metabolite composition of different colonic transit functions in FC. In addition to assessing clinical symptoms, this study aimed to better understand how intestinal flora contributed to impaired gut motility in FC patients. Results Significant microbiota taxonomic differences were observed across different gut dynamics in FC; Alistipes , Akkermansia , Oscillibacter , Ruthenibacterium , Alistipes_onderdonkii , and Ruthenibacterium_lactatiformans were key bacteria in FC patients with delayed colonic transit time; Roseburia and Klebsiella_pneumoniae were key bacteria in FC patients with normal colonic transit time; and Escherichia , Enterobacter , Escherichia_coli , Ruminococcus gnavus , Enterobacter_cloacae_complex , and Megamonas_funiformis were the key organisms in healthy controls. The metabolomics analysis revealed three differentially abundant short-chain fatty acids...