Combined effects of prolonged temperature and hypoxia stress on growth, metabolism and physiological status of yellow catfish (Pelteobagrus fulvidraco)

作者：Jie Wang, Liang Li, Shasha Zhao, Man Wang, Bo Li, Hongxi Guo, Dong Han, Xiaoming Zhu, Lei Zhang · 发表于：Aquaculture Reports · 年份：2025 · DOI：10.1016/j.aqrep.2025.102884 · 被引用次数：9 · 研究领域：Physiological and biochemical adaptations、Aquaculture Nutrition and Growth

Temperature and dissolved oxygen concentration are critical to fish. This study analyzed the effects of prolonged temperature and dissolved oxygen stress on growth metabolism, oxidative stress, immunity, and apoptosis in yellow catfish ( Pelteobagrus fulvidraco ). To this end, yellow catfish were continuously exposed to three temperatures (15, 26, and 32 °C) and two dissolved oxygen concentrations (2.5 and 6.5 mg/L) for 28 days. Notably, unsuitable temperatures and hypoxic exposure significantly decreased growth parameters (final body weight, weight gain, and specific growth rate), decreased survival rate, and significantly decreased crude protein and crude lipid in muscle ( p < 0.05). Further, the serum glucose content was reduced, lactate dehydrogenase activity and lactic acid content were increased, and the activities of key glycolysis enzymes (hexokinase, pyruvate kinase, and phosphofructokinase) were significantly elevated ( p < 0.05). Notably, prolonged temperature and dissolved oxygen stress triggered oxidative stress in yellow catfish, as evidenced by a significant increase in malondialdehyde content in serum and gills ( p < 0.05), elevated levels of antioxidant enzyme activity and expression (superoxide dismutase, catalase, and glutathione peroxidase), and damaged gills and head kidney tissues. In addition, the expression of immuno-inflammatory factors and apoptotic genes ( c3-like , tnfaip2 , il-1β , caspase-3 and caspase-9 ) was significantly increased (p < 0.05) i...