Exploration of gene presence/absence variations in Oncorhynchus mykiss and their differentiation between wild and selection populations

作者：Hancheng Bao, Na Xue, Boyuan Wang, Yu Han, Ming Huang, Jinghong He, Shuanglin Dong, Yangen Zhou, Qinfeng Gao, Yuan Tian · 发表于：Open Biology · 年份：2025 · DOI：10.1098/rsob.240382 · 被引用次数：3 · 研究领域：Genetic and phenotypic traits in livestock、Genetic diversity and population structure、Genetic Mapping and Diversity in Plants and Animals

Gene presence/absence variations (PAVs) have been considered as the important determinants of genome evolution and phenotypic diversity. However, studies on gene PAVs have been poorly documented, especially in fishes. In the present study, the pan-genome of rainbow trout was constructed based on 268 whole-genome re-sequencing accessions (4.38 Tb data). It recovered an additional 62 Mb sequences and 1288 protein-coding genes. Then, 9831 (22.77%) gene PAVs were genotyped across the 268 individuals. PAV-based PCA analysis, together with phylogenetic topology and STRUCTURE, revealed the clear separation among the different wild and selection populations. Additionally, a PAV-based genome-wide association study (GWAS) identified three candidate PAVs significantly associated with artificial selection. Meanwhile, fixation index analysis revealed 35 PAVs with significant frequency differences between wild and selection populations in Canada, while 15 candidate PAVs were detected between the populations in America. Their biological functions have been reported to participate in the regulation of growth performance and stress response. The present study deepens our understanding of widespread gene PAVs and facilitates the identification of key candidates that contribute to important traits.