Evidence for trans-generational immune priming against Vibrio splendidus in the oyster Crassostrea gigas

作者：Xiaorui Song, Weilin Wang, Miren Dong, Chang Liu, Chuanyan Yang, Lingling Wang, Linsheng Song · 发表于：Frontiers in Immunology · 年份：2025 · DOI：10.3389/fimmu.2025.1536562 · 被引用次数：5 · 研究领域：Aquaculture disease management and microbiota、Invertebrate Immune Response Mechanisms、Aquaculture Nutrition and Growth

Cumulative evidence have demonstrated the occurrence of trans-generational immune priming (TGIP) in invertebrates; however, the detailed substances transferred, and the mechanism of this transmission remain unclear. In the present study, we first tested TGIP in the offspring of Crassostrea gigas after parental challenge with Vibrio splendidus during the spawning season. In the maternal oyster primed with V. splendidus , the enzyme activities (lysozyme and SOD), NO level, the expression of immune genes ( CgTLR2 , CgMACPF , and CgFBG ), as well as the antibacterial activities were significantly enhanced in the eggs of V. splendidus -primed female oysters, indicating that V. splendidus stimulation promoted the immunity tendentiously transferred to eggs during the spawning season. After fertilization, the enzyme activities of CAT, lysozyme, and SOD were significantly enhanced in the maternal primed group [mVs-Sw (M)] during early oyster ontogeny, whereas there were no detectable differences between the control group (nSw-Sw (N)) and paternal primed group [pVs-Sw (P)]. However, the expression of immune genes ( CgGalectin , Cg Myd88 , and CgLBP ) increased in the parental primed groups (mVs-Sw (M) and pVs-Sw (P)). After the larval offspring were exposed to the secondary V. splendidus stimulation, the mortality rates in the parental TGIP groups [mVs-Vs (M1) and pVs-Vs (P1)] were significantly lower, while the hatching rates were significantly higher than those in the nSw-Vs (N1), co...