Multi-omics analysis of glycolytic reprogramming and ROS dynamics in host-specific responses to Salmonella Typhi infection in mice

作者：Yanrui Bai, Wenxiu Liu, Zhiyuan Liu, Dandan Ding, Huiya Jin, Shangyu Xiao, Jiayin Guo, Xiaoe He, Qian Wang, Han Xiao, Yan Wang, Tiansheng Zhang, Yana Li, Jing Yang, Hui Sun · 发表于：Journal of Advanced Research · 年份：2025 · DOI：10.1016/j.jare.2025.05.027 · 被引用次数：8 · 研究领域：Cancer Research and Treatments、Metabolomics and Mass Spectrometry Studies、Salmonella and Campylobacter epidemiology

INTRODUCTION: Salmonella Typhi (S. Typhi), a Gram-negative, serves as the etiological agent of typhoid fever. In contrast to other Salmonella serovars, S. Typhi exclusively infects humans. However, the molecular interactions it engages in with the host immune system remain inadequately characterized. This study adopts a multi-omics strategy to elucidate the immune and metabolic dynamics within the murine spleen during S. Typhi infection. OBJECTIVES: To identify and analyze transcriptomic, proteomic, and metabolomic alterations in the spleens of mice infected with S. Typhi. By comparing these host responses with those elicited by Salmonella Typhimurium (S. Typhimurium), a closely related serovar possessing a broad host range, the study seeks to uncover the unique metabolic reprogramming and immune-modulatory mechanisms specific to S. Typhi infection. METHODS: A multi-omics strategy was adopted, integrating transcriptomic, proteomic, and metabolomic data obtained from the spleen tissues of S. Typhi-infected mice. S. Typhimurium was utilized as a comparative control to distinguish host-specific responses. Additionally, the dynamics of reactive oxygen species (ROS), which play pivotal roles in mediating immune responses during infection, were examined. RESULTS: Integration of multi-omics datasets demonstrated distinct metabolic and immunological responses orchestrated by S. Typhi infection. Host metabolism was reprogrammed by S. Typhi through the upregulation of glycolysis and th...