Tissue-resident microbiota signature in nasopharyngeal carcinoma

作者：Xi-Rong Tan, Han Qiao, Ying-Qing Li, Wei Jiang, Sheng‐Yan Huang, Sha Gong, Wen‐Fei Li, Ling‐Long Tang, Guan‐Qun Zhou, Ye‐Lin Liang, Hui Li, Qing‐Mei He, Jiewen Bai, Mingliang Ye, Jing-Yun Wang, Sai-Wei Huang, Junyan Li, Chunqiao Gan, Ying‐Qin Li, Yin Zhao, Ying Sun, Jun Ma, Na Liu · 发表于：Microbiome · 年份：2025 · DOI：10.1186/s40168-025-02114-w · 被引用次数：13 · 研究领域：Gut microbiota and health、Head and Neck Cancer Studies、Cancer Immunotherapy and Biomarkers

Emerging evidence reveals that microbiota plays a crucial role in multiple cancers. Nasopharyngeal carcinoma (NPC) tissues harbour microbiota, highlighting the need to investigate the clinical implications of tissue-resident microbiota in the development of NPC. Here, we aim to clarify the specific profile of tissue-resident microbiota and its influence on NPC outcomes. This retrospective study included 491 NPC patients from Sun Yat-sen University Cancer Center (Guangzhou, China) and the Affiliated Hospital of Guilin Medical College (Guilin, China). We profiled the microbial composition of 343 NPC and 36 normal nasopharyngeal tissues through sequencing of the genes encoding the 16S rRNA subunit of bacterial ribosomes. There were significant differences in microbial composition, alpha diversity (Shannon index, P = 0.007; Simpson index, P = 0.036), and beta diversity (Bray–Curtis distance: R2 = 0.016, F = 5.187, P = 0.001; unweighted UniFrac distance: R2 = 0.017, F = 5.373, P = 0.001) between NPC and normal nasopharyngeal tissues. A bacterial signature comprising four risk bacterial genera, including Bacteroides, Alloprevotella, Parvimonas, and Dialister, was constructed in the training cohort (n = 171). Patients in the high-risk group had shorter disease-free (HR 2.80, 95% CI 1.51–5.18, P < 0.001), distant metastasis-free (HR 4.00, 95% CI 1.77–9.01, P < 0.001), and overall survival (HR 3.45, 95% CI 1.77–6.72, P < 0.001) than those of patients in the low-risk group. Similar res...