A Bimetallic Nanozyme Synergistic Effect-Driven Enzyme Cascade Nanoreactor for Instant Immunoassay

作者：Di Wu, Zhichao Yu, Jiao Qin, Mei‐Jin Li, Dianping Tang · 发表于：Analytical Chemistry · 年份：2025 · DOI：10.1021/acs.analchem.5c02358 · 被引用次数：88 · 研究领域：Advanced Nanomaterials in Catalysis、Advanced biosensing and bioanalysis techniques、Electrochemical sensors and biosensors

This study presents a colorimetric sensor for cancer screening utilizing the bifunctional enzyme activity of NiCo Prussian blue analogue (PBA), a PBA material. By introducing oxygen vacancies and employing a dual-metal doping strategy, NiCo PBA overcomes the limitations in catalytic activity observed in single-metal-doped materials (such as Ni PBA and Co PBA), significantly enhancing both peroxidase-like (POD) and catalase-like (CAT) activities. Compared to single-metal-doped Ni PBA and Co PBA, NiCo PBA exhibited a 30.08-fold increase in POD activity and a 4.83-fold increase in CAT activity, demonstrating higher sensitivity in carcinoembryonic antigen (CEA) detection. By integrating NiCo PBA with a cascade catalytic reaction principle, we developed a highly efficient and sensitive CEA detection method. NiCo PBA was utilized as a catalytic material in this method. Under the action of glucose oxidase, the decomposition of hydrogen peroxide was catalyzed by NiCo PBA, and oxygen was generated. Furthermore, a blue flocculent substance was produced when NiCo PBA was reacted with a chromogenic substrate. Through mutual verification by these two methods, the quantitative determination of CEA in serum samples was achieved. The experimental results demonstrated that the POD-like activity detection range was 0.2–50 ng mL –1, with a limit of detection (LOD) of 0.061 ng mL –1, while the CAT-like activity detection range was 0.1–20 ng mL –1, with an LOD of 0.028 ng mL –1 . The sensitivity ...