SM22α-Lineage Perivascular Stromal Cells Contribute to Abdominal Aortic Aneurysm

作者：Xiaoxi Pan, Run Zhang, Bingling Lu, Siyuan Chen, Hongjin Chen, M.-F. Li, Le Qin, Zhiyun Song, Yi Yang, Zhe Wang, Fuhua Yan, Aijun Sun, Fang Wu, Lichi Zhang, Ji‐Guang Wang, Tomasz J. Guzik, Pingjin Gao · 发表于：Circulation Research · 年份：2025 · DOI：10.1161/circresaha.124.325750 · 被引用次数：16 · 研究领域：Aortic aneurysm repair treatments、Cardiovascular Disease and Adiposity、Hippo pathway signaling and YAP/TAZ

BACKGROUND: Perivascular adipose tissue (PVAT) is a key regulator of vascular dysfunction. Impairment of PVAT phenotypic plasticity with aging may play a role in vascular pathology including abdominal aortic aneurysms (AAAs). Yet, the mechanisms underlying PVAT plasticity in aneurysm pathogenesis remain elusive. METHODS: Single-cell RNA sequencing was performed on perivascular stromal cells (PVSCs) from young (2- to 3-month-old) and aged (18- to 20-month-old) mice. The expression of PGC-1α (peroxisome proliferator-activated receptor gamma coactivator-1α) was measured in PVAT of aged mice and human aneurysm samples. Loss- and gain-of-function approaches were used to investigate the role of SM22α (Smooth Muscle 22-Alpha)-lineage PVSCs–derived PGC-1α in aneurysm development. Molecular mechanisms were explored through transcriptome and functional studies in young and aged mice, SM22α Cre ; Rosa26 RFP/+ ; PGC1α f/f and SM22α Cre ; Rosa26 RFP/+ mice with Ang II (angiotensin II)–induced and deoxycorticosterone acetate/salt-induced AAA models. RESULTS: SM22α + cells accumulated in PVAT of Ang II–treated aged mice and patients with aortic aneurysms. Single-cell RNA sequencing analysis revealed that aging disrupted the differentiation potential of SM22α-lineage PVSCs and led to reduced PGC-1α levels. PGC1α downregulation in PVAT was observed in both mouse AAA models and human aneurysm lesions. In mice with SM22α-driven PGC-1α deletion, Ang II–induced AAA formation was accompanied by pe...