Comparative transcriptome analysis reveals key genes associated with meiotic stability and high seed setting rate in tetraploid rice

作者：Pincang Lv, Man Wang, Rongde Qiu, Chang Yao, Meng Fang, Yuandong Xing, Xianhua Zhang, Yuchi He, Detian Cai, Zhaojian Song · 发表于：BMC Plant Biology · 年份：2025 · DOI：10.1186/s12870-025-06672-x · 被引用次数：3 · 研究领域：Chromosomal and Genetic Variations、Genetic Mapping and Diversity in Plants and Animals、Plant Molecular Biology Research

BACKGROUND: Polyploid rice has a high yield potential and excellent nutritional quality. The development of polyploid rice remained critically limited for several decades due to low seed setting rate until the successful breeding of polyploid meiosis stability (PMeS) lines. To determine the mechanism responsible for meiotic stability and high seed setting rate of PMeS line, agronomic traits, pollen fertility and viability, and meiotic behaviors of PMeS and non-PMeS lines were investigated. Further, comparative transcriptome analysis was performed to identify genes associated with meiotic stability and high seed setting rate in PMeS line. RESULTS: The seed setting rate, fertile and viable pollen ratios of PMeS line were significantly higher than those of non-PMeS line. The PMeS line exhibited stable meiosis, and chromosomes mainly paired as bivalents, rarely as univalents and multivalents in prophase I. Few lagging chromosomes were observed in anaphase I. By contrast, the homologous chromosomes pairing was disorganized in the non-PMeS line, with low frequencies of bivalents and high frequencies of univalents and multivalents in prophase I, while more cells with increased lagging chromosomes were detected in anaphase I. Many differentially expressed genes (DEGs) between PMeS and non-PMeS lines were identified through comparative transcriptome analysis. Some meiosis-related genes were specifically investigated from all DEGs. Further, several meiotic genes were identified as cand...