Enterotype-stratified gut microbial signatures in MASLD and cirrhosis based on integrated microbiome data

作者：Heng Yuan, Junyu Zhou, Xuangao Wu, Shiwei Wang, Sunmin Park · 发表于：Frontiers in Microbiology · 年份：2025 · DOI：10.3389/fmicb.2025.1568672 · 被引用次数：8 · 研究领域：Gut microbiota and health、Liver Disease Diagnosis and Treatment、Liver Disease and Transplantation

Introduction: Metabolic dysfunction-associated steatotic liver disease (MASLD) is a growing global health challenge, characterized by significant variability in progression and clinical outcomes. While the gut microbiome is increasingly recognized as a key factor in liver disease development, its role in disease progression and associated mechanisms remains unclear. This study systematically investigated the gut microbiota's role in MASLD and liver cirrhosis progression, focusing on individual bacterial strains, microbial community dynamics, and functional characteristics across different enterotypes. Methods: Publicly available next-generation sequencing(NGS) datasets from healthy individuals and patients with MASLD and cirrhosis were analyzed. Enterotype classification was performed using principal component analysis, with advanced bioinformatics tools, including Linear Discriminant Analysis Effect Size (LEfSe), eXtreme Gradient Boosting (XGBoost), and Deep Cross-Fusion Networks for Genome-Scale Identification of Pathogens (DCiPatho), to identify differentially abundant microbes and potential pathogens. Microbial co-occurrence networks and functional predictions via PICRUSt2 revealed distinct patterns across enterotypes. Results and discussion: were significantly associated with cirrhosis. Functional analysis revealed reduced biosynthesis of fatty acids, proteins, and short-chain fatty acids (SCFAs), coupled with increased lipopolysaccharide(LPS) production and altered seco...