LcTprxII Overexpression Enhances Physiological and Biochemical Effects in Maize Under Alkaline (Na2CO3) Stress

作者：David Pitia Julius Michael, Qing Liu, Yuejia Yin, Xuancheng Wei, Jianwei Lü, Faiz Ur Rehman, Aroge Temitope, Buxuan Qian, Hanchao Xia, Jiarui Han, Xiangguo Liu, Long Jiang, Xin Qi, Ruidong Sun, Ziqi Chen, Jian Zhang · 发表于：Plants · 年份：2025 · DOI：10.3390/plants14101467 · 被引用次数：4 · 研究领域：Plant Stress Responses and Tolerance、Photosynthetic Processes and Mechanisms、Redox biology and oxidative stress

Alkaline stress limits crop productivity by causing osmotic and oxidative damage. This study investigated the new gene LcTprxII, a type II peroxiredoxin encoded by Leymus chinensis, and its role in enhancing alkaline stress tolerance in transgenic maize. The gene was cloned, overexpressed, and characterized using RT-PCR, phylogenetic analysis, and motif identification. Transgenic maize lines were generated via Agrobacterium-mediated transformation and subjected to physiological, biochemical, and transcriptomic analyses under alkaline stress. Under alkaline stress, the results revealed that LcTprxII overexpression significantly preserved chlorophyll content, mitigated oxidative damage, and maintained growth compared to wild-type plants, as evidenced by elevated activities of antioxidant enzymes (APX, CAT, SOD, and POD) and reduced malondialdehyde (MDA) content. Transcriptomic profiling identified 3733 differentially expressed genes and the upregulation of ABA and MAPK signaling pathways, highlighting the role of these genes in stress signaling and metabolic adaptation. Hormonal analysis indicated reduced ABA and increased GA levels in the transgenic lines. This study identified WRKY, bHLH, and MYB transcription factors as key regulators activated under alkaline stress, contributing to transcriptional regulation in transgenic maize. Field trials confirmed the agronomic potential of LcTprxII-overexpressing maize, with yield maintained under alkaline conditions. The present study...