A Sensitive SERS Sensor for Simultaneous Detection of Two Potential Biomarkers of Alzheimer’s Disease: AChE and MAO-B

作者：Niya Yang, Xiaowan Zhang, Anran Liu, Yong Wang, Wei Wei · 发表于：Analytical Chemistry · 年份：2025 · DOI：10.1021/acs.analchem.5c01757 · 被引用次数：7 · 研究领域：Gold and Silver Nanoparticles Synthesis and Applications、Spectroscopy Techniques in Biomedical and Chemical Research、Advanced Nanomaterials in Catalysis

Monoamine oxidase B (MAO-B) and acetylcholinesterase (AChE) are potential biomarkers for Alzheimer’s disease (AD). However, few methods exist to detect both AChE and MAO-B simultaneously. In this study, we developed a SERS sensor for the simultaneous detection of AChE and MAO-B based on the one-pot reaction system, which provided more valuable information for early AD diagnosis. Au NPs modified with Raman reporter 4-aminothiophenol (Au PATP NPs) were attached to Cu-BTC via Schiff’s base reaction (Au PATP NPs@Cu-BTC), resulting in great Raman intensity. Phenethylamine (PEA), a substrate of MAO-B, competed with Au PATP NPs for binding to Cu-BTC, reducing the intensity of the Raman signal. Additionally, two PATP molecules on free Au NPs coupled to DMAB due to the catalysis of Cu 2+ in Cu-BTC, transforming Au PATP NPs into Au DMAB NPs and generating three new Raman peaks. TCh, the catalytic product of AChE, was chelated with Cu 2+, so the coupling efficiency of PATP has been decreased, and the conversion of Au PATP NPs to Au DMAB NPs has been prevented. Based on the one-pot reaction system, both MAO-B and AChE can be detected by Raman signals from precipitated Au PATP NPs@Cu-BTC and supernatant Au DMAB NPs after centrifugation. The detection limits were 2.3 × 10 –3 μg mL –1 for MAO-B and 1.6 × 10 –3 U L –1 for AChE. We successfully detected MAO-B and AChE in serum with recoveries ranging from 100.0 to 113.7% for MAO-B and 93.6 to 120% for AChE. This manuscript presents a novel me...