Enhanced prevention on postoperative atrial fibrillation by using anti-inflammatory biodegradable drug patch

作者：Pengcheng Yu, Weiqi Lu, Huaxin Sun, Chengchen Huang, Xiao-Lin Zhou, Yuxing Wang, Zhen Zhang, Guosheng Fu, Hanxiong Liu, Ke‐feng Ren, Xia Sheng · 发表于：Regenerative Biomaterials · 年份：2025 · DOI：10.1093/rb/rbaf040 · 被引用次数：4 · 研究领域：Antiplatelet Therapy and Cardiovascular Diseases、Microbial Metabolism and Applications

Abstract Postoperative atrial fibrillation (POAF) is the most prevalent form of secondary atrial fibrillation and increases the risk of adverse cardiovascular outcomes, such as stroke, heart failure and increased mortality. Herein, we designed an andrographolide (Andr)-loaded degradable polymer patch to deliver the drug directly to the atrial tissue for prevention of POAF. The sterile pericarditis (SP) rat model was adopted for highly relationship to clinical practice. The patch-released Andr effectively reduced the incidence of atrial fibrillation from 90 to 20%, and alleviated local atrial inflammation and oxidative stress in vivo, by using electrophysiological detection and histological analysis such as immunofluorescence, western blot and PCR. In HL-1 cells, we found the use of Andr-loaded patch could strongly inhibit the cell death, reactive oxygen species (ROS) generation and mitochondrial injury caused by LPS. Meanwhile, the use of Andr-loaded patch could effectively inhibited macrophages polarize towards M1. Mechanistically, we verified that the regulation of the cytoplasm and mitochondria Ca2+ and ROS dynamic balance was quite important both in vivo and in vitro. Our strategy proved by regulating the inflammatory microenvironment, ROS balance and Ca2+ homeostasis and the Andr-loaded atrial patch was effective for POAF in the SP rat model. The electrical signal of atrial stromal reentry in the case of this model was successfully mined, and the results of calcium chann...