A New Ribo-Type of Wangodinium sinense from Germination of Resting Cysts Isolated from Ballast Tank Sediments of Incoming Ships to China

作者：Zhe Tao, Caixia Yue, Yuyang Liu, Shuo Shi, Ruoxi Li, Zhaoyang Chai, Yunyan Deng, Lixia Shang, Zhangxi Hu, Haifeng Gu, Fengting Li, Ying Zhong Tang · 发表于：Journal of Marine Science and Engineering · 年份：2025 · DOI：10.3390/jmse13050942 · 被引用次数：4 · 研究领域：Protist diversity and phylogeny、Marine Toxins and Detection Methods、Microbial Community Ecology and Physiology

In recent decades, ships’ ballast water and associated sediments have been recognized globally as significant vectors for the dissemination of non-indigenous species, which has attracted extensive attention due to its ecological and economic impacts. The characteristics of production of resting cysts in the dinoflagellate life cycle further increases the risk of biological invasions through ballast tank sediments. Despite extensive research which has characterized the species diversity of dinoflagellate cysts within ballast tank sediments, the possibility and importance of invasions caused by different ribosomal types of the same species have been paid little attention. In this study, two cultures of dinoflagellates were established through cyst germination from the ballast tank sediments collected from two ships (“THETIS” and “WARIYANAREE”) arriving at the Jiangyin Port (China) and identified as Wangodinium sinense Z. Luo, Zhangxi Hu, Yingzhong Tang and H.F. Gu by comprehensive phylogenetic analysis of rDNA sequences (including LSU, SSU, and ITS1-5.8S-ITS2). Despite the rDNA sequences of the isolates showing a generally high similarity to reference sequences, the LSU D1-D6 sequences contained up to 11 stable single nucleotide polymorphisms (SNPs), while SSU and ITS1-5.8S-ITS2 sequences exhibited up to five and two divergence sites, respectively. Moreover, phylogenetic analyses based on partial LSU and SSU rDNA sequences further indicated that strains germinated from ships’ b...