The virome of the panglobal, wide host-range plant pathogen Phytophthora cinnamomi : phylogeography and evolutionary insights

作者：Leticia Botella, Ondřej Hejna, Tomáš Kudláček, Kateřina Kovačiková, M. Rost, Marco Forgia, Milica Raco, Ivan Milenković, Tamara Corcobado, C. M. B. de F. Maia, Bruno Scanu, A. Drenth, David Guest, Edward C. Y. Liew, Nguyen Minh, Phạm Quang Thu, Tun‐Tschu Chang, Fu ChuenHsu, Koji Kageyama, Ayaka Hieno, Hayato Masuja, Seiji Uematsu, Álvaro Durán, Marthin Tarigan, Muhammad Junaid, Nasri Nasri, Eugenio Sanfuentes, Vladislav Čurn, Joan Webber, C. M. Brasier, Marília Horta Jung, Thomas Jung · 发表于：Virus Evolution · 年份：2025 · DOI：10.1093/ve/veaf020 · 被引用次数：6 · 研究领域：Plant Virus Research Studies、Plant and Fungal Interactions Research、Plant Disease Resistance and Genetics

Abstract Phytophthora cinnamomi stands out as one of the most devastating plant pathogens worldwide, rapidly expanding its range and impacting a wide range of host species. In this study, we investigated the virome of P. cinnamomi across 222 isolates from Africa, Asia, Europe, Oceania, and the Americas using stranded total RNA sequencing, reverse transcription polymerase chain reaction screening, and Sanger sequencing of selected isolates. Our analysis revealed that virus infections were prevalent across all sampled populations, including RNA viruses associated with the orders Ghabrivirales, Martellivirales, and Tolivirales, and the classes Amabiliviricetes, Bunyaviricetes, and the recently proposed Orpoviricetes. Viruses were mainly found in East and Southeast Asian populations, within the geographic origin of P. cinnamomi but have also spread to new regions where the pathogen has emerged as a clonal destructive pathogen. Among the identified viruses, eight species, including two bunya-like viruses, one narna-like virus, and five ormycoviruses, exhibit a global distribution with some genetic divergence between continents. The interaction between P. cinnamomi and its virome indicates a dynamic coevolution across diverse geographic regions. Indonesia is indicated to be the viral epicentre of P. cinnamomi, with the highest intra- and interspecies diversity of viruses. Viral diversity is significantly enhanced in regions where sexual recombination of P. cinnamomi occurs, while r...