Therapeutic effects and mechanisms of Fufang Longdan mixture on metabolic syndrome with psoriasis via miR-29a-5p/IGF-1R axis

作者：Guangyun Luo, Xiangyi Kong, Fang Wang, Zhiming Wang, Zhuo Zhang, Huan Cui, Yiwen Zhang, Wen Huang, Xuesong Yang, Jianzhou Ye · 发表于：Frontiers in Pharmacology · 年份：2025 · DOI：10.3389/fphar.2025.1585369 · 被引用次数：3 · 研究领域：Psoriasis: Treatment and Pathogenesis、Metabolomics and Mass Spectrometry Studies、Mangiferin and Mango Extracts

Background The occurrence of comorbid metabolic syndrome and psoriasis (MS-P) is owing to the complex interplay between metabolic dysregulation and inflammatory responses. However, current treatments have shown limited efficacy in improving the symptoms of both conditions simultaneously. Objective This study aimed to investigate the therapeutic efficacy of Fufang Longdan Mixture (FLM) in treating MS-P comorbidity, elucidate its mechanism through the miR-29a-5p/IGF-1R axis and evaluate treatment responses between APOE −/− and C57BL/6 mice. Methods UPLC-Q-exactive-MS/MS analysis was used to characterise FLM’s chemical composition. Metabolic syndrome was induced in APOE −/− and C57BL/6 mice using a high-fat, high-sugar diet, while psoriasis-like lesions were induced in the mice via the administration of imiquimod. The mice were randomised into control, model, Yinxieling (8 g/kg/d) and FLM (0.5 mL/d) groups. We assessed the treatment efficacy through metabolic parameters, hematoxylin and eosin (H&E) staining and inflammatory cytokine profiling. The direct targeting of IGF-1R by miR-29a-5p was verified via dual-luciferase reporter assays. We analysed the expression patterns and interactions of miR-29a-5p/IGF-1R using RT-qPCR, Western blotting and fluorescence in situ hybridisation. Results Chemical analysis identified 2,665 compounds in FLM, which were predominantly shikimates and phenylpropanoids (32%), alkaloids (20%) and terpenoids (13%). FLM significantly improved meta...