Antioxidant activity of low molecular weight polysaccharides from Tremella fuciformis in Caenorhabditis elegans based on metabolomics analysis

作者：Quancen Lee, Zhixiang Xue, Mingfeng Zheng, Bin Liu, Feng Zeng · 发表于：Journal of Future Foods · 年份：2025 · DOI：10.1016/j.jfutfo.2025.04.012 · 被引用次数：10 · 研究领域：Genetics, Aging, and Longevity in Model Organisms、Seaweed-derived Bioactive Compounds、Biochemical Acid Research Studies

Tremella fuciformis polysaccharides have rich bioactivities, while low molecular weight polysaccharides have higher bioavailability and nutritional value. In this study, it was investigated that the structural characteristics of low molecular weight polysaccharides from Tremella fuciformis (TFLP) prepared by acid hydrolysis method, its impact on antioxidant capacity and stress resistance of Caenorhabditis elegans ( C. elegans ), and potential mechanisms. TFLP was an acidic polysaccharide mainly composed of mannose and xylose with a molecular weight of 106 kDa. TFLP could prolong the median lifespan of C. elegans under heat stress and acute oxidative stress conditions through significantly reducing the MDA level and increasing the activity of antioxidant enzymes T-SOD, GSH Px and CAT. The intervention of high-dose TFLP significantly prolonged the median lifespan of C. elegans under heat stress and acute oxidative stress conditions by 24.47% and 7.84%, respectively. At the same time, the MDA levels significantly decreased by 69.59%, and the levels of antioxidant enzymes T-SOD, GSH-Px and CAT increased significantly by 2.22, 1.28 and 0.53 times, respectively. Meanwhile, the transcription levels of daf-16, fat-5, fat-7 , and hsf-1 mRNA in C. elegans treated with TFLP were significantly increased, while the transcription levels of akt-1 and daf-2 mRNA were significantly reduced. In addition, propanoate metabolism, valine, leucine and isoleucine degradation metabolism and vitamin B...