Multi-omics analysis reveals immunosuppression in oesophageal squamous cell carcinoma induced by creatine accumulation and HK3 deficiency

作者：Yingzhen Gao, Siyu He, Xiaoyan Meng, Kun Zheng, Heyang Cui, Yikun Cheng, Xinyuan Shen, Yuanfang Zhai, Binbin Zou, Fang Wang, Hongyi Li, Pengzhou Kong, Yanqiang Wang, Xuefei Feng, Bin Yang, Ruifang Sun, Yongsheng Meng, Enwei Xu, Yanlin Guo, Ning Ding, Wei-Min Zhang, Xiaolong Cheng, Lunzhi Dai, Yongping Cui, Ling Zhang · 发表于：Genome Medicine · 年份：2025 · DOI：10.1186/s13073-025-01465-1 · 被引用次数：8 · 研究领域：Ferroptosis and cancer prognosis、Cancer, Hypoxia, and Metabolism、Immune cells in cancer

BACKGROUND: Deep insights into the metabolic remodelling effects on the immune microenvironment of oesophageal squamous cell carcinoma (ESCC) are crucial for advancing precision immunotherapies and targeted therapies. This study aimed to provide novel insights into the molecular landscape of ESCC and identify clinically actionable targets associated with immunosuppression driven by metabolic changes. METHODS: We performed metabolomic and proteomic analyses combined with previous genomic and transcriptomic data, identified multi-omics-linked molecular features, and constructed metabolic-immune interaction-based ESCC classifiers in a discovery cohort and an independent validation cohort. We further verified the molecular characteristics and related mechanisms of ESCC subtypes. RESULTS: Our integrated multi-omics analysis revealed dysregulated proteins and metabolic imbalances characterizing ESCC, with significant alterations in metabolites and proteins linked to genetic traits. Importantly, ESCC patients were stratified into three subtypes (S1, S2, and S3) on the basis of integrated metabolomic and proteomic data. A robust subtype prediction model was developed and validated across two independent cohorts. Notably, patients classified under the poorest prognosis subtype (S3 subtype) exhibited a significant immunosuppressive microenvironment. We identified key metabolism-related biomarkers for the S3 subtype, specifically creatine and hexokinase 3 (HK3). Creatine accumulation an...