The alleviating effects and mechanisms of Enterococcus faecium Kimate-X and Lactobacillus plantarum Kimate-F combination on canine inflammatory bowel disease

作者：Rui Zhang, Wanjin Hu, Saiwei Zhong, Weiyang Chen, Shuang Xie, Mei‐Ru Chen, Qinghua Yu · 发表于：Frontiers in Veterinary Science · 年份：2025 · DOI：10.3389/fvets.2025.1534665 · 被引用次数：4 · 研究领域：Gut microbiota and health、Probiotics and Fermented Foods、Clostridium difficile and Clostridium perfringens research

Introduction Canine inflammatory bowel disease (IBD) is characterized by chronic intestinal inflammation and dysbiosis. Conventional treatments often result in adverse effects and contribute to antibiotic resistance, highlighting the need for safe, effective alternatives. Probiotics have gained attention for their potential in modulating gut microbiota and immune responses. This study investigates the therapeutic mechanisms of Enterococcus faecium Kimate-X and Lactobacillus plantarum Kimate-F, individually and in combination, in alleviating canine IBD. Methods In vitro antibacterial and anti-inflammatory activities were assessed using agar well diffusion assays and LPS-induced RAW 264.7 macrophages, respectively. In vivo efficacy was evaluated in dextran sulfate sodium (DSS)-induced colitis models in mice and dogs. Metagenomic sequencing was performed on canine fecal samples to analyze microbiota composition and functional pathways. Short-chain fatty acid (SCFA) levels were quantified, and key host signaling pathways were examined. Results Kimate-F showed strong antibacterial effects against Escherichia coli , Salmonella enteritidis , and Yersinia enterocolitica . Kimate-X significantly suppressed nitric oxide (NO) and TNF-α production in the in vitro inflammation model. In both mouse and canine DSS-induced colitis models, the probiotic combination significantly reduced weight loss, colonic damage, and serum inflammatory cytokines, while increasing IL-10 levels. Metagenomic a...