LOX5 roles in forming diverse aroma profiles of Nicotiana tabacum via multi-omics analysis

作者：Chen Dong, Longyun Cao, Feng Li, Linjie Song, Meng Chen, Peng Jia, Yulong Gao, Xuefen Wang, Lifeng Jin · 发表于：Industrial Crops and Products · 年份：2025 · DOI：10.1016/j.indcrop.2025.121141 · 被引用次数：6 · 研究领域：Plant biochemistry and biosynthesis、Plant Gene Expression Analysis、Microbial Metabolic Engineering and Bioproduction

s As the global cash crops, the formation of distinct aroma profiles in Nicotiana tabacum have garnered significant research interest. Lipids as precursors of aroma compounds in plants, play an essential role in defining the unique aroma characteristics of tobacco. In this study, lipidomics analysis was performed to identify a total of 471 lipid components in leaves of strong fragrance tobacco (SF) and mild fragrance tobacco (MF). Comparative analysis of SF and MF revealed 70 differentially changed lipid compounds, with 26 up-regulated and 44 down-regulated lipids. The up-regulated lipids were predominantly glycoglycerolipids (GL) and glycerophospholipids (GP), while the down-regulated lipids were enriched in sphingolipids (SP) and fatty acyls (FA). Gas chromatography-mass spectrometry (GC-MS) revealed that SF exhibited relatively higher levels of cis-3-hexenal and cis-3-hexenol compared to MF. Transcriptomics analysis identified a total of 905 differential expression genes (DEGs), with 370 up-regulated and 535 down-regulated DEGs. Notably, a robust correlation was observed between the expression of the key gene lipoxygenase 5 (LOX5) and content of phosphatidylcholine (PC) (15:0_18:3), a precursors of linolenic acid. Additionally, a positive correlation was detected between LOX5 expression and the degradation products of linolenic acid, including cis-3-hexenal and cis-3-hexenol. Transient over-expression of LOX5 in tobacco leaves resulted in an enhanced accumulation of these ...