Bacterial Community in Sugarcane Rhizosphere Under Bacillus subtilis Inoculation and Straw Return

作者：Francisco de Alcântara Neto, Danielly Araújo Pinheiro, Sandra Mara Barbosa Rocha, Marcos Renan Lima Leite, Romário Martins Costa, Janderson Moura da Silva, Sabrina Hermelindo Ventura, Mayanna Karlla Lima Costa, Thâmara Kelly dos Santos Apollo Sousa, Rafael de Souza Miranda, Karolayne Ribeiro Caetano, Érika Valente de Medeiros, Arthur Prudêncio de Araújo Pereira, Lucas William Mendes, Ademir Sérgio Ferreira de Araújo · 发表于：Soil Systems · 年份：2025 · DOI：10.3390/soilsystems9020044 · 被引用次数：6 · 研究领域：Plant-Microbe Interactions and Immunity、Biofuel production and bioconversion、Genomics and Phylogenetic Studies

Straw return enhances soil biological properties by increasing carbon and energy availability, thereby improving conditions for microbial communities. However, the introduction of beneficial bacteria, such as Bacillus subtilis, can further shape the rhizosphere bacterial composition. In this study, we combined sugarcane straw return with B. subtilis inoculation to test whether this synergy reduces microbial specialization in the sugarcane rhizosphere. Three treatments were evaluated: (I) bulk soil (bulk), (II) rhizosphere soil with straw return but no B. subtilis inoculation (straw), and (III) rhizosphere soil with straw return and B. subtilis inoculation (straw + Bacillus). The bacterial community, including plant-growth-promoting bacteria (PGPB), was analyzed via 16S rRNA amplicon sequencing. Neither straw return nor B. subtilis inoculation significantly altered bacterial richness, diversity, or phylum-level abundance in the rhizosphere. Actinobacteria, Firmicutes, and Proteobacteria dominated the community, with Bacillus, Bradyrhizobium, and Paenibacillus as the predominant PGPB genera. Notably, only Bradyrhizobium abundance increased in the rhizosphere when straw was co-applied with B. subtilis. A co-occurrence network analysis revealed stronger microbial interactions under straw return, while B. subtilis enhanced connectivity among the PGPB. Although niche occupancy remained stable, PGPB specialization was higher with straw alone, suggesting that B. subtilis fosters a mo...