Metabolic Response of Black Tiger Shrimp (Penaeus monodon) to Acute Ammonia Nitrogen Stress

作者：Yangyang Ding, Shigui Jiang, Song Jiang, Yundong Li, Qibin Yang, Lishi Yang, Jianhua Huang, Jianzhi Shi, Pengying Li, Hongshan Diao, Falin Zhou · 发表于：Biology · 年份：2025 · DOI：10.3390/biology14050501 · 被引用次数：9 · 研究领域：Physiological and biochemical adaptations、Aquaculture Nutrition and Growth、Aquaculture disease management and microbiota

High concentrations of ammonia nitrogen could result in the death of aquatic animals and cause a huge economic loss in the aquaculture industry. However, the metabolic responses to acute ammonia nitrogen stress remain largely unknown in Penaeus monodon. In this study, we first investigated the histological change in tissues in Penaeus monodon under 96 h acute ammonia nitrogen stress. The result of the paraffin section showed that acute ammonia nitrogen stress induced severe epithelial detachment and lumen dilatation of the hepatopancreas, swollen and hemocyte infiltration of the gills, and mucosa exfoliation and shortened villi of the intestine in Penaeus monodon, suggesting the impairment of the normal physiological function in these tissues. We next examined the change in the metabolic product in the plasma and the enzyme activity in the hepatopancreas after ammonia nitrogen stress. Upon ammonia stress, both the concentration of ammonia and urea nitrogen significantly increased, while there was no significant increase in the concentration of uric acid, which is consistent with the results that the enzyme activity of glutamine synthetase (GS), glutamate dehydrogenase (GDH), and aspartate transaminase (GOT) became significantly elevated and the enzyme activity of adenosine deaminase (ADA) in the purine metabolism pathway significantly decreased after ammonia stress, suggesting that shrimp could convert excessive ammonia to urea for ammonia detoxification through the ammonia–n...