Antifungal Activity and Potential Mechanisms of Two Bafilomycin Analogues Isolated from Streptomyces sp. NEAU-Y11 against Colletotrichum orbiculare

作者：Wenshuai Song, Xiaoyan Yu, Xiaoxin Yu, Haifeng Zhang, Kuan Zhang, Lifeng Guo, Jidong Wang, Donglan Tian, Qingtao Yu, Xiangjing Wang, Junwei Zhao, Wensheng Xiang · 发表于：Journal of Agricultural and Food Chemistry · 年份：2025 · DOI：10.1021/acs.jafc.5c03728 · 被引用次数：7 · 研究领域：Plant Pathogens and Fungal Diseases、Plant-Microbe Interactions and Immunity、Plant Disease Resistance and Genetics

Cucumber anthracnose, caused by Colletotrichum orbiculare, severely affects the cucumber yield and quality. In this study, two active compounds, bafilomycin C 1 and JBIR-100, were isolated from strain NEAU-Y11 and exhibited strong antifungal activity against C. orbiculare, with EC 50 values of 0.0491 and 0.1042 μg/mL, respectively, significantly lower than those of the commercial fungicide (4.42 μg/mL). Pot experiments demonstrated effective control of cucumber anthracnose at 0.2 μg/mL for bafilomycin C 1 and 0.4 μg/mL for JBIR-100, with efficacies reaching 78.5 and 67.7%, respectively. Microscopy and biochemical analyses indicated that both compounds disrupted the fungal cell wall, membrane, and redox homeostasis, leading to cell death. Transcriptome analysis further revealed the effects of bafilomycin C 1 ′s on amino acid metabolism, cell structure, redox homeostasis, and DNA double-strand break repair. These findings suggest that bafilomycin C 1 and JBIR-100 are promising candidates for use as agrochemical fungicides to control C. orbiculares and may serve as a basis for developing next-generation antifungal agents.