Enhanced oral bioavailability of two Cistanche polysaccharides in acteoside: an in-depth analysis of intestinal flora, short-chain fatty acids, and pharmacokinetic regulation

作者：Jing Lian, Yuan Zhang, Kexu Dong, Ji Shi, Fan Zhang, Guoshun Shan, Pengpeng Liu, Nan Wang, Tianzhu Jia · 发表于：Frontiers in Nutrition · 年份：2025 · DOI：10.3389/fnut.2025.1509734 · 被引用次数：8 · 研究领域：Phytochemistry and Biological Activities、Polysaccharides and Plant Cell Walls、Medicinal Plants and Bioactive Compounds

Introduction For centuries, Cistanche deserticola Y. C. Ma has been considered to have the effect of “tonifying the kidney and strengthening the yang,” and is used for the prevention and treatment of diseases such as impotence, female infertility, lumbago and senile constipation. Polysaccharides and small molecules of acteoside are the main chemical compounds co-existing in Cistanche deserticola Y. C. Ma with health benefits, but the interaction of these two compounds in vivo is not yet known. Methods Here, we investigated the effects of unprocessed Cistanche polysaccharides and wine-processed Cistanche polysaccharides on the metabolism of acteoside in vivo through gut microbiota. Male Sprague–Dawley rats were randomly divided into Control group, unprocessed Cistanche polysaccharide group (UCP group) and wine-processed Cistanche polysaccharide group (WCP group). After 21 days of intervention with unprocessed Cistanche polysaccharides and wine-processed Cistanche polysaccharides, rats were given 100 mg/kg of acteoside on day 22. Acteoside and its associated metabolites pharmacokinetically studied were analysed using UPLC-QqQ-MS, and the composition of short-chain fatty acids (SCFAs) in excrement was measured using the technique of GC–MS. The microbiological composition of the intestines was discovered using 16S rRNA gene amplicon sequencing. Results The results showed that the Cistanche polysaccharides used in this experiment, including unprocessed Cistanche polysaccharides an...