LIPUS Responsive Dopamine-Modified PVDF Piezoelectric Nanofiber Membrane for Full-Thickness Skin Wound Healing

作者：Zhiying Zhang, Lijun Liu, Huan Wang, Wangni Xie, Wenhao Zhai, Linlin Wen, Boya Zhang, Kexuan Liu, Xue Zhang, Shu‐Chen Liu, Lei Huang, Daowei Li, Yanmin Zhou · 发表于：International Journal of Nanomedicine · 年份：2025 · DOI：10.2147/ijn.s496921 · 被引用次数：17 · 研究领域：Wound Healing and Treatments、Planarian Biology and Electrostimulation、Advanced Sensor and Energy Harvesting Materials

Objective: Large full-thickness skin wounds pose significant challenges, particularly in achieving scar-free healing and the regeneration of skin appendages. This study introduces a portable approach for promoting scarless healing and skin appendage regeneration by utilizing low-intensity pulsed ultrasound (LIPUS) activated piezoelectric electrospun membranes to modulate the local electrical environment. Methods: Dopamine-modified polyvinylidene fluoride (DA/PVDF) nanomembranes were fabricated via electrospinning, followed by piezoelectric characterization under varying LIPUS stimulation. Cell adhesion was examined using SEM and laser confocal microscopy to assess surface interactions. Cell proliferation and migration were further analyzed using the CCK-8 assay and Transwell migration assay, respectively. Finally, the effects of DA/PVDF membranes on full-thickness skin defect healing were tested in a mouse model. The healing process was documented with photographs, and functional skin regeneration was evaluated through histological analysis. Results: The DA/PVDF nanomembranes had an average diameter of 732 ± 232 nm and generated a voltage of 450 mV under LIPUS stimulation, a 1.28-fold increase compared to PVDF membranes alone. In vitro, LIPUS-activated membranes enhanced cell adhesion and proliferation, resulting in a 1.14-fold increase in cell growth over three days. The transwell migration assays showed 244.67 ± 7.85 migrated cells. In vivo , the DA/PVDF+LIPUS group exhibit...