Follow-up of antibody changes in brucellosis patients in Gansu, China

作者：Hanyu Sha, Qun Duan, Dongyue Lyu, Fang Qian, Xiaojin Zheng, Jiazhen Guo, Zhaokai He, Xinmin Lu, Asaiti Bukai, Shuai Qin, Ran Duan, Saier Guli, Peng Zhang, Meng Xiao, Huaiqi Jing, Xin Wang · 发表于：Microbiology Spectrum · 年份：2025 · DOI：10.1128/spectrum.02862-24 · 被引用次数：9 · 研究领域：Brucella: diagnosis, epidemiology, treatment、Zoonotic diseases and public health、Bartonella species infections research

, often leads to chronic infection with recurrent symptoms, posing significant therapeutic challenges. Effective monitoring of antibody dynamics is crucial for understanding disease progression and enhancing clinical outcomes. To investigate the longitudinal dynamics of antibody titers and antigen-specific responses in animal husbandry practitioners exposed to brucellosis in Gansu, China. We conducted a serological study involving 400 animal husbandry workers, identifying 21 seropositive individuals (5.25%). Antibody titers and antigen-specific responses to lipopolysaccharide (LPS) and BP26 protein were assessed using the Rose-Bengal test (RBT), serum agglutination test (SAT), and western blotting. Acute brucellosis patients displayed a distinct three-phase antibody titer pattern: a rapid rise (12-38 days), a rapid decline (19-41 days), and a slow decline (42-148 days). BP26-specific antibodies persisted significantly longer than LPS antibodies, remaining detectable up to 395 days post-infection. Early treatment reduced acute-phase antibody titers, correlating with disease control. This study combined dynamic antibody titer monitoring with specific antigen monitoring, enhancing the accuracy and specificity of brucellosis diagnosis and offering a basis for chronic infection detection. The findings are highly significant for clinical practice and public health strategies, particularly in high-incidence areas, prevention and control, as well as high-risk population surveillance....