Proximity-induced saccharide binding to a protein's active site within a confined cavity of coordination cages

作者：Takahiro Nakama, Miri Tadokoro, Risa Ebihara, Maho Yagi‐Utsumi, Koichi Kato, Makoto Fujita · 发表于：Chemical Science · 年份：2025 · DOI：10.1039/d5sc00782h · 被引用次数：7 · 研究领域：Chemical Synthesis and Analysis、Enzyme Structure and Function、Supramolecular Self-Assembly in Materials

-fold decrease in the apparent dissociation constant of the monosaccharide. The significant enhancement of the saccharide binding to the lysozyme active site effectively inhibited its enzymatic activity. NMR studies confirmed the formation of lysozyme-saccharide complexes through enhanced weak interactions, which are otherwise unobservable, facilitated by the confined cavity. This cage confinement strategy thus offers a novel approach for ligand-based functional control of native proteins, eliminating the need for elaborate ligand design and protein engineering.