Isolation, identification, whole genome sequence analysis, and pathogenicity of a potential recombinant goose parvovirus

作者：Qinghe Zhu, Huinan Li, Hansong Li, Wenfei Bai, Jingxuan Zhou, Ming Liu, Yingying Zhao, Limin Jiang, Ying Sun, Jia Sun, Jingjing Zhao, Jia Hu, Chunqiu Li, Xiaoxu Xing, Dan Yang, Dongbo Sun · 发表于：Poultry Science · 年份：2025 · DOI：10.1016/j.psj.2025.105231 · 被引用次数：6 · 研究领域：Virus-based gene therapy research、Animal Virus Infections Studies、Bacteriophages and microbial interactions

Goose parvovirus (GPV) is the etiological agent responsible for gosling plague (GP), which is an acute hemorrhagic infectious disease affecting geese, posing significant economic challenges to the poultry industry. Furthermore, recent studies have identified that the novel goose parvovirus (NGPV), a recombinant variant of the classic GPV, is responsible for duck short beak dwarfism syndrome, which has significantly affected duck farm. Therefore, the infection and genetic evolution of GPV have attracted widespread attention of researchers in poultry disease. In order to clarify the prevalence and genetic evolution of clinically severe GPV in the Heilongjiang region, this study successfully isolated a strain of GPV HLJ2023 from goose embryos, which results in the mortality rate of 100 % after 5 generations. The electron microscope shows that the virus particles are spherical, with a diameter of approximately 28 nm, and HLJ2023 strain has a total genome length of 5048 nt. SimPlot analysis showed that HLJ2023 strain is closely related to duck parvovirus and NGPV in the VP3 gene region. Recombination analysis showed that the isolated strain is a potential recombinant of the NGPV JS191021 strain and the GMD (Goose parvovirus hosted by Muscovy duck) PT strain. the strong pathogenicity of HLJ2023 strain to goslings. 36 h after the challenge, the goslings were depressed and had a mortality rate up to 100 %. Autopsy revealed intestinal bleeding, thinning of the intestinal wall, and a l...