Establishment and application of high‐pressure propagation breeding (HPPB)‐mediated genetic transformation system in citrus rootstocks

作者：Siyu Zhang, Rui‐Fang Luo, Wu Yuan, Tingting Zhang, Abdulhamid Yusuf, Nian Wang, Min Li, Shuo Duan · 发表于：Plant Biotechnology Journal · 年份：2025 · DOI：10.1111/pbi.70072 · 被引用次数：7 · 研究领域：Plant tissue culture and regeneration、Plant-Microbe Interactions and Immunity、Plant Pathogens and Fungal Diseases

Citrus cultivation plays a pivotal role in global agriculture and food security. With intensifying international market competition and increasing environmental challenges, citrus crops have become particularly urgent (Mukhametzyanov et al., 2024). Traditional breeding and genetic transformation are two main strategies for improvement, with the latter gaining more attention due to its ability to introduce specific traits that are difficult to achieve through conventional methods (Gutierrez-E et al., 1997). Citrus rootstocks are crucial for enhancing fruit quality, disease resistance, and stress tolerance; their root systems are not only vital for water and nutrient uptake but also help establish beneficial connections with soil bacteria (Song et al., 2023). Genetic transformation technology offers tremendous potential for improving citrus crops without altering the genetic background of the scion (Cheng et al., 2021). This technology enhances rootstock quality, disease resistance, reduces pesticide use, improves fruit safety, and boosts market competitiveness (D'Amico et al., 2018; Zhang et al., 2022). Moreover, the improved rootstock root systems can better adapt to adverse environments, promote the proliferation of beneficial microorganisms, and enhance soil fertility and structure. Despite the labor-intensive, time-consuming, and contamination-prone nature of traditional transgenic root production methods, recent research suggests that cutting plants and encouraging rootin...