Exploring the active ingredients of Banzhilian and its mechanism of action on diabetic Gastric cancer based on network pharmacology

作者：Haikun Cao, Hui Song, Weiguo Zhou, X. Lv, Xinlei Liu, Zheng Xiang, Rui Fu, Yixian Cheng, Junjie Chen, Shengwei Wang, Yanping Hu, Huayue Yan, Wenlong You, Changqian Guo, Bo Chen, Guodong Cao, Dawei Wang, Jianguang Jia · 发表于：Scientific Reports · 年份：2025 · DOI：10.1038/s41598-025-98214-6 · 被引用次数：1 · 研究领域：Pharmacological Effects of Natural Compounds、Traditional Chinese Medicine Analysis、Ginseng Biological Effects and Applications

The incidence of Gastric cancer (GC) has shown a sharp upward trend, and patients with GC complicated by diabetes exhibit significantly worse clinical outcomes and prognosis compared to those without diabetes. Traditional Chinese medicine has played a crucial role in the treatment of both GC and diabetes. Currently, Banzhilian(Scutellaria barbata D. Don) is utilized in the treatment of GC; however, the specific small-molecule monomers it contains and their mechanisms of action have not yet been fully elucidated. This study aims to explore the mechanism of quercetin, a key component of Banzhilian, through network pharmacology, molecular docking, molecular dynamics (MD) simulation, bioinformatics, and in vitro and in vivo experiments. Initially, core targets and key pathways involved in the treatment of diabetes-associated GC (GC-diabetes) were identified using public databases. Subsequently, molecular docking, MD simulation, and survival analysis were performed. Experimental validation included CCK-8 assays, colony formation assays, apoptosis detection, cell cycle analysis, wound healing assays, Transwell migration assays, Western blotting, and mouse subcutaneous tumor formation experiments to evaluate the effects of quercetin, as an active monomer in Banzhilian, on Gastric cancer cells (HGC-27-HG cells) under high-glucose conditions. In this study, quercetin was identified as the key active component, with AKT1, TP53, JUN, MYC, and CCND1 recognized as the target genes, and th...